Public Health
Dengue Detection Kit
Multiplex real-time PCR for differential detection of DENV-1, 2, 3 and 4.
Format
Multiplex real-time PCR
Manufactured in
Jeddah · KSA
Quality system
ISO 13485 aligned
GMP
WHO GMP aligned
Overview
The Gene Matrix Dengue Detection Kit is a multiplex real-time PCR assay designed for differential detection and serotyping of all four dengue virus serotypes (DENV-1, DENV-2, DENV-3, DENV-4) — supporting clinical diagnosis, public-health surveillance, and Hajj-season outbreak response.
Clinical Context
- Dengue is endemic in several regions of the Kingdom — particularly the western coastal provinces — with seasonal peaks that align with mass-gathering events.
- Differential serotyping is clinically important: secondary infection with a different serotype is a recognised risk factor for severe dengue.
- Locally manufactured surveillance assays strengthen the Kingdom's outbreak-readiness and Hajj public-health response.
Target Panel — Four Dengue Serotypes
| Parameter | Detail |
|---|---|
| Serotypes detected | DENV-1 · DENV-2 · DENV-3 · DENV-4 |
| Differentiation | Serotype-specific channels |
| Internal control | Endogenous human gene control |
| Sample type | Serum / plasma |
| Format | Multiplex real-time PCR |
Workflow
From sample to result, in four steps
- 01
Sample collection
Validated transport medium and sample types per assay specification.
- 02
Nucleic acid extraction
Compatible with major automated and manual extraction workflows.
- 03
Real-time PCR
Multiplex amplification on standard 96-well thermocyclers.
- 04
Reporting
Genotype/serotype-specific channels for clear clinical reporting.
Performance
Validated to international references
Each assay is characterised for analytical sensitivity, specificity and limit of detection against established clinical references. Full validation packs and lot-specific data are shared with prospective customers under appropriate confidentiality.
Analytical sensitivity
Validated against international reference panels
Analytical specificity
No cross-reactivity with related pathogens
Limit of detection
Lot-specific data on request
Frequently asked
Does the assay distinguish primary from secondary dengue infection?
The assay is a molecular detection and serotyping test. Distinguishing primary vs secondary infection requires complementary serology — we are happy to discuss complete laboratory workflows.
Is the assay suited to Hajj-season surveillance?
Yes. The differential-serotype design is specifically valuable for surge-capacity surveillance during mass-gathering events.
What is the typical turnaround time?
Turnaround is comparable to standard real-time PCR workflows. Specific timings depend on extraction platform and laboratory throughput.